Project

Part:BBa_K1857002:Design

Designed by: Joshua Timmons   Group: iGEM15_Northeastern_Boston   (2015-09-16)


HSP70A-RBCS2_Hyg Expression Plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplified the pHSP70A-RBCS2-Intron region with the iGEM prefix/suffix added to the ends. We then amplified 3'UTR RBCS2 with PstI upstream' and SpeI downstream. The hygromycin B resistance cassette was then amplified with XbaI upstream and SpeI downstream. pSB1C3 was amplified with primers which removed ligated with digested pHSP70A-RBCS2-Intron and pSB1C3 for final assembly.


Source

The parts were adapted from pOpt_mVenus_Hyg.

References